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. 2013 Sep 27;8(9):e75643. doi: 10.1371/journal.pone.0075643

Figure 1. BB0347 is expressed and produced in culture.

Figure 1

A) Expression of BB0347 was verified by RT- PCR with flaB mRNA as a control. Lane 1: flaB from genomic DNA, lane 2: flaB from cDNA, lane 3: no RT control, lane 4: bb0347 from genomic DNA, lane 5: bb0347 from cDNA, lane 6: no RT control, L: ladder. B) Western blotting shows that BB0347 protein is produced in the spirochete at all sampled time points. C) QRT- PCR of bb0347 at two different temperatures of incubation with flaB as a standard. D) Western blot using αBB0347 and αFlaB against whole-cell lysates from spirochetes grown at either 34 or 23°C to similar cellular densities. All figures are representative of at least two independent experiments with similar results, and error bars indicate ±SEM.