Figure 2. Recombinant BB0347 binds Fn in a dose-dependent manner.

Fn-binding was determined by ELISA using BSA (negative control), RevA (positive control), and BB0347 as coating proteins. Fn was added to wells (0, 10, and 20 µg/mL) allowed to bind, and any unbound Fn was washed away. Values were blanked against the negative control to eliminate background from non-specific binding. BB0347 bound Fn in a dose-dependent manner as determined by an increased binding of αFn antibodies in wells treated with BB0347. Results are representative of three independent experiments–of at least 4 replicates for each concentration–with similar results, and error bars are indicative of ±SEM. Significance was determined by comparing the values of each concentration of Fn to the 0 Fn control values for both proteins after blanking with the BSA negative control.