Figure 3. rBB0347-Fn binding is partially dependent on ionic interactions.

BB0347 binding to Fn was measured via ELISA as in Fig. 2; except that varying levels of several inhibitors were used. Both NaCl (A) and heparin (B) inhibited the binding of BB0347 to Fn, while the addition of the lysine analogue 6-ACA (C) was not found to have any effect. All values were blanked against BSA. Results are representative of at least three independent experiments with similar results, and at least 4 replicates for each concentration. Error bars indicate ±SEM.