Table 1. Primers used in this study.
Name | Sequence (5′–3′) | Use | |
Fla3* | GGGTCTCAAGCGTCTTGG | Amplification of flaB for QRT-PCR | |
Fla4* | GAACCGGTGCAGCCTGAG | ||
CAB105 | ACATTGAAAACGAAAAGGAA | Amplification of bb0347 for QRT-PCR | |
CAB106 | AAAGAATTTTTGCCCTTTTT | ||
BB0347protF | CACCATGATAAAAATGTCTTTGAATTACACTG | Cloning of BB0347 into pET200 | |
BB0347protR | TTAGGTTTGATTTTTTATTTTTTTTATTAG | ||
OspCprotF | CACCTGTAATAATTCAGGGAAAG | Cloning of OspC into pET200 | |
OspCprotR | TTAAGGTTTTTTTGGACTTTCTG | ||
FlaBprotF | CACCATTATCAATCATAATACATCAGCTA | Cloning of FlaB into pET200 | |
FlaBprotR | TTATCTAAGCAATGACAATGACAAAACATATTGG |
All primers were designed during the course of this study, except Fla3 & 4, which have been described previously [44].