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. 2013 Sep 27;8(9):e75643. doi: 10.1371/journal.pone.0075643

Table 1. Primers used in this study.

Name Sequence (5′–3′) Use
Fla3* GGGTCTCAAGCGTCTTGG Amplification of flaB for QRT-PCR
Fla4* GAACCGGTGCAGCCTGAG
CAB105 ACATTGAAAACGAAAAGGAA Amplification of bb0347 for QRT-PCR
CAB106 AAAGAATTTTTGCCCTTTTT
BB0347protF CACCATGATAAAAATGTCTTTGAATTACACTG Cloning of BB0347 into pET200
BB0347protR TTAGGTTTGATTTTTTATTTTTTTTATTAG
OspCprotF CACCTGTAATAATTCAGGGAAAG Cloning of OspC into pET200
OspCprotR TTAAGGTTTTTTTGGACTTTCTG
FlaBprotF CACCATTATCAATCATAATACATCAGCTA Cloning of FlaB into pET200
FlaBprotR TTATCTAAGCAATGACAATGACAAAACATATTGG
*

All primers were designed during the course of this study, except Fla3 & 4, which have been described previously [44].