Figure 2. N-termini of anaerobic energy generation enzymes in A. castellanii, showing putative mitochondrial targeting peptides (mtTPs).

Putative mtTPs were predicted by TargetP, and are shown underlined and bold. N-termini of bacterial and eukaryotic homologues are shown for comparison; bacterial homologues lack targeting peptides. Positively charged residues in the predicted mtTPs are shown in red; hydrophobic residues are shown in blue. Arginine residues at positions −2, −3 or −10, and hydrophobic residues at position −8, believed to be important in determining the cleavage site [66], are marked with an asterisk. P, TargetP mitochondrial targeting probability.