Figure 4. Effect of streptochlorin on expression of FcεRI in RBL-2H3 cells and in vitro activity of Lyn and Fyn.

The activity of Lyn (A) and Fyn (B) was measured by the ADP-Glo™ kinase assay. The values indicate mean ± standard error of the mean from three independent experiments by one-way analysis of variance followed by Dunnett's multiple comparison test. * P<0.05, ** P<0.01, and *** P<0.001 significant difference from the substrate-alone group. (C) RBL-2H3 cells were treated with or without IgE and streptochlorin for 1 h. The cells were then collected and analyzed by flow cytometry for expression of FcεRI. Representative images are shown from three independent experiments. Str, streptochlorin.