Figure 7. Effects of streptochlorin on IgE-mediated PCA reaction in mice.

Upon antigen challenge, leakage of injected Evans blue into the ear was significantly reduced in streptochlorin-injected (i.v.) mice. Representative pictures of the ear are shown (A). Vascular leakage assayed by Evans blue extravasation into ear tissue. The same size ears were excised 20 min after i.v. challenge with antigen and Evans blue and subsequently extracted in formamide (B). Each column shows mean optical density at 650 nm. The values indicate mean ± standard error of the mean (n = 3), from three independent experiments by one-way analysis of variance followed by Dunnett's multiple comparison test. ** P<0.01 and *** P<0.001 significantly different when compared with α-DNP IgE and DNP-HSA treated groups. Str, streptochlorin.