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. 2013 Sep 27;8(9):e75212. doi: 10.1371/journal.pone.0075212

Figure 3. Titre and reactivity of phages rescued by AGM13 and VCSM13.

Figure 3

(A) The phagemid clones rescued with AGM13 and VCSM13 were titrated on E. coli TG1 and scored as the number of Ampr transductants (CFU/ml); 3301 scFv is an antibody fragment specific to the protein MPT64 encoded by the M. tuberculosis gene Rv1980c; 19 kDa antigen is encoded by the M. tuberculosis gene Rv3763; and MAb 1905 is a monoclonal antibody against the 19 kDa antigen. (B) Different dilutions of rescued phages were added to Maxisorb™ plates coated with MPT64 (for 3301 scFv-displaying phages) or MAb 1905 (for 19 kDa antigen-displaying phages). The bound phages were detected using a HRP conjugated anti-phage MAb. Values are mean ± SD of three independent experiments. For Fig. 3B error bars are too small to be visible as compared to the physical size of the symbol.