Table 1. Trypsin sensitivity assay.
Trypsin concentration(µg/ml) | PFU/ml | |
AGM13 | VCSM13 | |
0.0 | 4.6×1012 | 5.4×1012 |
0.1 | 3.4×106 | 3.2×1012 |
1.0 | 2.6×106 | 3.0×1012 |
10.0 | 2.6×103 | 2.2×1012 |
100.0 | 0 | 1.6×1012 |
Purified phages (2×1012) were treated with trypsin in total volume of 1.0 ml for 30 min at 37°C. The phages were titrated by infecting E. coli TG1 and the numbers of PFU were scored.