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. 2013 Sep 27;8(9):e75212. doi: 10.1371/journal.pone.0075212

Table 1. Trypsin sensitivity assay.

Trypsin concentration(µg/ml) PFU/ml
AGM13 VCSM13
0.0 4.6×1012 5.4×1012
0.1 3.4×106 3.2×1012
1.0 2.6×106 3.0×1012
10.0 2.6×103 2.2×1012
100.0 0 1.6×1012

Purified phages (2×1012) were treated with trypsin in total volume of 1.0 ml for 30 min at 37°C. The phages were titrated by infecting E. coli TG1 and the numbers of PFU were scored.