Table 2. Analysis of random clones at various stages of library construction.
| Stage No. | Stage | I. Aligning to M. tb genome* | II. ORF in-frame with PelBss (and gIIIP)† | III. Aligning to M. tb proteome‡ |
| A. MTBLIB25 Library (100–300 bp) | ||||
| 1. | MTBLIB25 C01 | 96.8 (92/95) | 1.05 (1/95) | – |
| 2. | MTBLIB25 P01 | 100 (47/47) | 2.1 (1/47) | – |
| 3. | MTBLIB25 C02 | 100 (48/48) | 89.6 (43/48) | 46.5 (20/43) |
| 4. | MTBLIB25 P02 | 100 (48/48) | 93.7 (45/48) | 55.5 (25/45) |
| B. MTBLIB27 Library (300–800 bp) | ||||
| 1. | MTBLIB27 C01 | 97.8 (90/92) | 1.1 (1/92) | 100 (1/1) |
| 2. | MTBLIB27 P01 | 100 (46/46) | 2.2 (1/46) | – |
| 3. | MTBLIB27 C02 | 100 (45/45) | 95.5 (43/45) | 53.5 (23/43) |
| 4. | MTBLIB27 P02 | 100 (46/46) | 97.8 (45/46) | 64.4 (29/45) |
Randomly selected clones were analyzed from (A) MTBLIB25 library (100–300 bp) and (B) MTBLIB27 library (300–800 bp) at various stages of library construction: 1, transformants obtained after large-scale electroporation of the ligation sample; 2, transductants obtained after infection of TOP10F’ cells with the primary phage library; 3, transductants obtained after infection of TOP10F’ cells with the trypsin-treated primary phages; 4, transductants obtained after infection of TOP10F’ cells with the secondary ORF-selected phage library.
Percentage of recombinant clones that aligned to the M. tuberculosis (M. tb) genome.
Percentage of clones in-frame with the PelB signal sequence (PelBss) and gIIIP in the phagemid.
Percentage of total in-frame clones (as in III) that aligned with the M. tb proteome (genic ORFs).
Number of positive clones/total clones analyzed is given in brackets.