Table 3. Panning of whole genome fragment libraries on monoclonal antibodies raised against mycobacterial proteins.

Monoclonal Antibody	MAb Ag85-12						MAb 1912
Rounds	Pan II				Pan III		Pan II				Pan III
Library	MTBLIB25		MTBLIB27		MTBLIB27		MTBLIB25		MTBLIB27		MTBLIB27
	Test	Control	Test	Control	Test	Control	Test	Control	Test	Control	Test	Control
Input phage	1×109	1×109	1×109	1×109	1×109	1×109	1×109	1×109	1×109	1×109	1×109	1×109
Output phage	3×106	8×104	3×105	3×104	1×106	3×104	1×106	7×105	9×105	2×105	1×106	6×104
Fold enrichment*	37.5		10.0		33.3		1.43		4.5		17
% Specific clones (no. of positive/no. analyzed)† #	77.7 (35/45)		71.9 (23/32)		90.6 (29/32)		(0/47)		12.5 (4/32)		68.7 (22/32)

Control and Test refer to wells coated with phosphate buffer saline (pH 7.2) and specific monoclonal antibody, respectively.

^*Fold enrichment = output phage in test/output phage in control.

^†The inserts in clones from test wells selected after panning were sequenced and aligned to the respective M. tuberculosis protein.

^#All the clones analyzed were in frame with resepect to PelBss and gIIIP.