Figure 3.

Schematic of a magnetic cell separation chip. An array of thin nickel stripes is deposited and patterned on a glass substrate to produce micro field gradients. A sample containing target cell-bead complex, non-target cells, beads, and buffer are introduced into the chip. The target cell-bead complex and unattached beads are trapped at the edges of the nickel stripes while the non-target cells flow to a waste collection tube. A washing buffer is then flowed through the chip to dislodge and elute unbound non-target species.