Skip to main content
. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: J Am Chem Soc. 2013 Aug 22;135(35):12924–12927. doi: 10.1021/ja403007r

Figure 2.

Figure 2

Fluorescence of modified DHFRs (0.5 µM), measured at pH 8.0. Green line is the modified DHFR containing biphenyl-phenylalanine (1) at position 17 (excitation at 280 nm); red line is the modified DHFR I containing biphenyl-phenylalanine (1) at position 17 and L-(7-hydroxycoumarin-4-yl)ethylglycine (2) at position 115 (excitation at 280 nm); black line is the modified DHFR containing L-(7-hydroxycoumarin-4-yl)ethylglycine (2) at position 115 (excitation at 280 nm); cyan line is the modified DHFR containing L-(7-hydroxycoumarin-4-yl)ethylglycine (2) at position 115 (excitation at 340 nm).