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. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: J Am Chem Soc. 2013 Aug 22;135(35):12924–12927. doi: 10.1021/ja403007r

Figure 3.

Figure 3

Fluorescence of modified DHFRs (0.5 µM), measured at pH 8.0. Fluorescence emission of modified DHFRs following excitation at 280 nm. Green line is the singly modified DHFR containing biphenyl-phenylalanine (1) at position 115; red line is the doubly modified DHFR II containing L-(7-hydroxycoumarin-4-yl)ethylglycine (2) at position 17 and biphenyl-phenylalanine (1) at position 115; black line is the singly modified DHFR containing L-(7-hydroxycoumarin-4-yl)ethylglycine (2) at position 17.