Skip to main content
. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Alcohol Clin Exp Res. 2013 Jun 25;37(10):1657–1667. doi: 10.1111/acer.12139

Figure 3.

Figure 3

Real-time RT-PCR analysis indicates that at 48 hpf, ethanol exposed zebrafish that show mild anatomical defects (< 2 scored defects) exhibit a significant increase (fold change relative to the mean of controls) in miR9 expression, whereas severely affected animals (>3 scored defects) exhibited significantly decreased miR-9 expression compared to non exposed controls. Inset graph shows ΔCT relative to U6 snRNA. Lower ΔCTs indicate higher levels of miRNA expression compared to higher ΔCTs. Data expressed as mean ± SEM, n = 3–7/group. *, p<0.05; ***, p<0.0001.