MiR-9 is expressed in the fetal telencephalon during mammalian development, and in neural and non-neural tissue during zebrafish development. A) DAPI stain localizes to nuclei. B) MiR-9 is expressed in the ventricular zone of the fetal murine brain at gestational day 12.5. C) Image of cultured neurospheres derived from the GD 12.5 mouse ventricular zone and cultured ex vivo. D–E) DAPI staining to visualize nuclei (D) and nonsense control (E) and in the fetal murine brain at GD 12.5, scale bar indicates 40 um. Abbreviations, LV, Lateral ventricle; VZ, ventricular zone. Scale bars, 40 um;