Pre-mir-9 transcripts accumulate following chronic ethanol exposure in mammals. A and B) Following 70mM ethanol exposure, miR-9-3 exhibits significant accumulation, while miR-9-2 exhibits a near-significant increase in accumulation. In both paradigms, miR-9-1 levels remained unaffected by ethanol. Asterisks indicate statistically significant comparisons of ΔCT, p<0.05 (lower ΔCT indicates increased expression of pre-miRNA), n=4–5/group. Data from acute and chronic ethanol treatment conditions were combined because, while there was a main effect of ethanol exposure on Pre-miR-9 accumulation, there was not a statistically significant effect of time.