Table 1.

Methylation-specific PCR primer sequence

Primer set	Forward primer, 5′→3′	CpG di-nucleotides	Reverse primer, 5′→3′	CpG di-nucleotides
MSP-9-1-M	CGT TTT TTT GAG GTT TCG TC	3	GTA ATC TCG AAT CGC TCG AC	2
MSP-9-1-U	TTT TGT TTT TTT GAG GTT TTG TT		TCC ATA ATC TCA AAT CAC TCA AC
MSP-9-2-M	GCG TTA AGG AGG TAA AAG GTC	2	TAA CGA CGA CGA CAA CAA C	3
MSP-9-2-U	TTA GTG TTA AGG AGG TAA AAG GTT		TAA CAA CAA CAA CAA CAA CAA C
MSP-9-3-M	TAT TCG TCG GTA TCG TTG TC	4	CTC TAA CTC TCC GCG TAC G	3
MSP-9-3-U	TTT TAT TTG TTG GTA TTG TTG TT		CCC CTC TAA CTC TCC ACA TAC A