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. 2002 Nov 6;71(6):1285–1295. doi: 10.1086/344515

Figure 4.

Figure 4

Characterization of the high-molecular-weight factor H associated with the R1210C mutation. a, 10% SDS-PAGE of the factor H (fH) proteins purified from patient HUS29 and control individual N3. Samples were reduced with 10% β-mercaptoethanol. A gel stained with 1% Coomassie is shown. b, Western blot analyses of reduced and nonreduced factor H proteins purified from patient HUS29 and control individual N3, using a rabbit anti-HSA polyclonal antibody. c, SDS-PAGE analysis of the wild-type (H29R; flow through) and mutated (H29C; retained) factor H proteins purified from patient HUS29, using anti-HSA affinity chromatography. Samples were not reduced. A silver-stained 10% polyacrylamide gel is shown.