PSC secrete inflammatory cytokines. Culture supernatants from the 7 primary PSC lines and the pancreatic fibroblast line, HPF, were collected when cells were 70% confluent. A, a panel of cytokines and chemokines were quantitated via Luminex analysis. Data are presented as a heat map for expression of soluble factors in supernatants from each cell line. B, chart indicates the levels of TH2 (IL-4 and IL-10) and TH1 (IFN-γ) cytokines observed in the Luminex bioplex (n.d., not detectable).