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Results from the analysis of two sperm with the insertion (+) haplotype and two with the deletion (−) haplotype. The three groups of primers used in the second round are indicated. M1, M2, and M3-1/M3-2 are mixtures of PCR products amplified with a single pair of primers that belong to the three groups. M is pBR322 DNA digested with MspI and is used as a molecular size marker. Missing bands are indicated with arrows.
