Fig. 1.
(A) Schematic of mouse Cyp2r1 genomic sequence depicting the null allele design and genotyping strategy. All 5 exons of Cyp2r1 gene on chromosome 7, indicated by numbered boxes, were replaced with β-galactosidase coding sequence (lacZ) and neomycin selection cassette (neor) to create a Cyp2r1−/− allele. Genotyping primers are indicated by arrows (Materials and Methods). (B) Agarose electrophoresis visualized by ethidium bromide staining showing Cyp2r1+/+, Cyp2r1+/−, and Cyp2r1−/− genotypes. Tissues from ear punch were used as DNA sources. PCR product sizes are 399 bp for wild-type and 245 bp for Cyp2r1−/−. M, DNA marker.