UV lignin stains of the seeds from BTX (S. bicolor, nonshattering) and HAL (S. halepense, shattering), showing parts of the seed structure, pedicel, glume, and abscission layer (AL). T4 mature stage of seeds from BTX and HAL, (A) unstained and (B) lignin stained. Various development stages (T1 through T4, in progressive maturity level: T1, early histo-differentiation; T2, embryonic cell expansion; T3, endosperm filling; T4, preabscision desiccation) of seeds from HAL, (C) unstained and (D) lignin stained. (Scale bar, 1 mm.)