Fig. 3.

The arminin family of antimicrobial peptides. (A) Phylogenetic analysis of the arminin AMP family from four different Hydra species. The tree was built by Bayesian interference of phylogeny. A total of 3 million generations were calculated using the general time reversible model and the invgamma rate variation and four chains with a burn-in of 25%. Posterior probabilities are shown at the corresponding nodes. Genes are colored according to species. Numbers indicate contig numbers in the species transcriptome. AEP, H. vulgaris (AEP); Hmag, H. magnipapillata; Holi, H. oligactis; Hvir, H. viridissima. (B) Relative expression of each arminin, compared with the expression of β-actin in the corresponding species. Expression data were retrieved from microarray data, conducted in three biological replicates. Bar charts represent mean + SD. Certain paralogues had no corresponding microarray probes (n.d., not detected).