Fig. 2.

Identification of interaction partners of Cdk1. (A) Quantification of Cdk1–prey protein interactions. Death index is calculated as the log₁₀ of the ratio of pixel mean intensities of 5-FC–treated divided by untreated colonies (5-FC/not treated). The Cdk1–Cdc19 interaction was found to be a technical false positive (see text). (B) Estimation of the FNR for 37 proteins identified to interact with Cdk1 by OyCD PCA (details of calculation in SI Text). (C) Examples of Cdk1 interacting partners with cyclin binding motifs and with (Lte1 and Mft1) or without (Rim20) consensus Cdk1 phosphorylation sites. Filled bars are high-quality sites (P ≤ 0.01, open bars are low-quality sites).