Figure 3.

Threshold of membrane tension in MscCG. (A) Currents of MscCG in the PB113 strain (ΔmscS and ΔmscK). Four-second pressure pulses with various amplitudes were applied to the patch membrane at a pipette potential of +40 mV. Current generated by MscCG was elicited at a low pressure, and current generated by MscL was activated at a higher pressure. The numbers in the current and pressure traces correspond to each other. (B) Relationship between pressure and the maximum current. P_mid of MscCG and MscL was determined by fitting to Boltzmann functions. The ratio of P_mid of MscCG to P_mid of MscL was 0.56 ± 0.05 (n = 4).