Figure 7.

Inactivation of MscCG and MscS. (A) Responses to two 2-s supramaximal pulses (2 × P_mid) separated by 30-s pressure. The intervening pressure was increased by 0.2 × P_mid. The numbers in the current and pressure traces correspond to each other. (B) The ratio of the currents generated by the first and second pulses is plotted as the inactivation rate of MscCG (solid circles) and MscS (open circles; n = 5). (C) Current response of Q112G S113G mutant of MscCG to the paired-pulse protocol. All data were taken in the MJF612 strain in 200 mM K-glutamate and the base salt mix at a pipette potential of +40 mV. Bars show SD.