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. 2013 Sep 17;105(6):1383–1387. doi: 10.1016/j.bpj.2013.08.003

Figure 4.

Figure 4

Cholesterol depletion from and replenishment of influenza virions. (A) Relative cholesterol depletion from X:31 influenza virions as a function of MβCD treatment. Error bars are plotted as the SD over four independent measurements per condition. Initial cholesterol levels were measured at 0.15 ± 0.0043 and 0.22 ± 0.0153 ng cholesterol per microgram of viral protein for batches A and B, respectively. (B) Results of cholesterol replenishment experiments after extraction with 0, 20, or 50 mM MβCD. (C) Results of lipid-mixing efficiency experiments on cholesterol-extracted and replenished virus. Extraction with 20 mM MβCD is fully reversible, whereas extraction with 50 mM MβCD is partially reversible. Values and error bars represent the mean and SD calculated from four to six independent experiments per condition in panel B and seven to 11 independent experiments per condition in panel C.