Figure 2.

Parental origin determination by PCR analysis using polymorphic markers on somatic cell hybrids. Shown are two markers used to determine the parental origins of the chromosomes involved in this case of a de novo rob(14q15q). The top of the figure shows the idiograms for chromosomes 14 (left), 15 (right), and the rob(14q15q) (middle). The left of the figure shows the results for D14S267 on DNA from the mother (M), child (C) with the de novo rob(14q15q), father (F), somatic cell hybrid carrying the rob(14q15q) (rob), and somatic cell hybrid carrying the free-lying chromosome 14 (fl). The child inherited the upper allele from the father and the lower allele from the mother. The chromosome 14 involved in the rob(14q15q) was derived from the mother, since it shares this lower allele. The free-lying chromosome 14 was inherited from the father. The right of the figure shows the results for D15S979. The child inherited the upper allele from the father and the lower allele from the mother. The chromosome 15 involved in the rob(14q15q) was derived from the father, as it carries the father’s allele. The free-lying chromosome 15 was inherited from the mother. Thus, this rob(14q15q) was derived postzygotically from the maternally derived 14 and the paternally derived 15.