Fig. 1.

Yeast mitochondria form a dynamic reticulum. Wild-type yeast cells producing mitochondrion-targeted green fluorescent protein (mito-GFP) were grown to log phase in media containing galactose at 30°C A 3 μl aliquot was placed on a microscope slide under a coverslip and sealed with nail polish. (a) Stereo pair of a mito-GFP-labeled, unbudded yeast cell. Three-dimensional images were acquired using wide-field fluorescence microscopy. (b) Time-resolved images from a single optical section of 0.2 μm. Time increases from left to right in 3 min increments as indicated. Arrows on the 3 and 9 min images mark fusion events; the arrow on the 21 min image marks a fission event. Bar, 2 μm.