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. 2013 Nov 5;368(1629):20130006. doi: 10.1098/rstb.2013.0006

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© 2013 The Author(s) Published by the Royal Society. All rights reserved.

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Figure 4. — Dynamics of Bem1-GFP polarization in diploids. (a,b) Clustering of Bem1-GFP in rsr1Δ/rsr1Δ (a, DLY9200) and RSR1/RSR1 (b, DLY9201) cells. Top panels are cropped images of polarization sites at 45 s intervals. t = 0 is 45 s before the first detection of polarized signal. Middle panels show the intensity changes of Bem1-GFP in the cluster. The trace ends at bud emergence. Bottom panels are traces of four other cells. (c) The fraction of Bem1-GFP that is polarized at the peak frame (mean ± s.e.m.) in rsr1Δ/rsr1Δ (DLY11780) and RSR1/RSR1 (Wt; DLY9201) cells (n = 10). Representative images are shown at top. (Online version in colour.)