Figure 5.

Fluctuating Bem1-GFP clusters. A subset of RSR1/RSR1 cells (DLY9201) initially display a low-level recruitment of Bem1-GFP, which then coalesces to a tighter cluster before bud emergence. Bottom panels: quantification of localized Bem1-GFP signal. Representative frames of cropped images show the fluctuating (top left) and coalescing stages (top right) in two individual cells. Residual Bem1-GFP signal at the previous cytokinesis site is also visible at the bottom of the cropped frames. (Online version in colour.)