Figure 2.

Collective invasion of Par3-depleted mammary cancer cells. Invasion assay chambers were established with clusters of tumour cells on top of a three-dimensional extracellular matrix pad in serum-free medium (SFM). Cells invaded towards the lower chamber with serum containing medium (SCM). Invading tumour cell clusters expressing NICD and either a control shRNA or Par3 shRNA were fixed and stained for E-cadherin and DNA. A representative cluster is shown. Scale bar, 10 µm. (Online version in colour.)