Table 2.
Primers designed from putative xylanase gene sequences ofA. nigerCBS 513.88
| Gene | Size (bp)a | Primer name | Primer sequence |
|---|---|---|---|
| xynI | 543 | xynIF | 5′-atgttcttcaagaccatcctt |
| xynIR | 5′-ttaacccagagtaagagtgaa | ||
| xynII | 686 | xynIIF | 5′-atgaaggtcactgcagcttt |
| xynIIR | 5′-taagaagatatcgtgacactg | ||
| xynIII | 746 | xynIIIF | 5′-atgctcaccaagaaccttct |
| xynIIIR | 5′-ttactgaacagtgatggacg | ||
| xynIV | 755 | xynIVF | 5′-tggtcgcctactcgtctc |
| xynIVR | 5′-tagcagctctcctcggtg | ||
| xynV | 826 | xynVF | 5′-atggtgtctttccttggcc |
| xynVR | 5′-ctatgcactaacggtgaagt | ||
| xynVI | 1043 | xynVIF | 5′-atgtcgcacccccaacag |
| xynVIR | 5′-ctacgataaagtcctcccc | ||
| xynVII | 1494 | xynVIIF | 5′-atggttcagatcaaggtagc |
| xynVIIR | 5′-ctagagagcatttgcgatag | ||
| xynVIII | 2962 | xynVIIIF | 5′-tgcgcgtaccgaaccgg |
| xynVIIIR | 5′-tcatatgctccgatgtccc | ||
| xynVIII2Fb | 5′-aacggaggcatacctcaac | ||
| xynVIII2Rb | 5′-tgttgccacccagcagac |
aPutative intron sequences are included in gene sizes.
bxynVIII2F and xynVIII2R were designed from the internal sequence of xynVIII.