Table 3.
Purification step | Total act. (U) | Total protein (mg) | Specific act. (U mg–1) | Yield (%) | Purification fold |
---|---|---|---|---|---|
Crude enzyme | 4,500 | 65 | 69 | 100 | 1.0 |
Lyophilization | 4,900 | 34 | 140 | 110 | 2.0 |
Anion-exchange (DE52) | 670 | 6.8 | 99 | 15 | 1.4 |
Purification step | Total act. (U) | Total protein (mg) | Specific act. (U mg–1) | Yield (%) | Purification fold |
---|---|---|---|---|---|
Crude enzyme | 4,500 | 65 | 69 | 100 | 1.0 |
Lyophilization | 4,900 | 34 | 140 | 110 | 2.0 |
Anion-exchange (DE52) | 670 | 6.8 | 99 | 15 | 1.4 |