Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Basic Res Cardiol. 2012 Nov 9;108(1):310. doi: 10.1007/s00395-012-0310-4

Figure 1.

Figure 1

Characterization of EPCs and upregulation of chemokine receptor expression and angiogenic chemokine/mediator secretion in EPCs. a Characterization of the EPCs used in this study. Flow cytometric analysis for CD11b, CD31, VEGFR-2, CXCR2, and CXCR4 surface expression on EPCs and eEPCs (fluorescence intensity of control IgG staining shown in shaded grey). b Effect of hypoxic treatment (2 % O2) on the surface expression levels of CXCR2, CXCR4, and CD74 on eEPCs. Normoxic conditions (20 % O2) (green) were compared with a 24 h (blue) and 48 h (red) hypoxic treatment and isotype control IgG staining (black). c The secretion of angiogenic EPC-derived factors/chemokines is upregulated by hypoxia. Secretion of MIF, VEGF, CXCL12, and CXCL1 from eEPCs at different time points following hypoxic stimulation was measured by ELISAs (*p < 0.05 vs. control, n = 3)

Simian virus 40-immortalized murine endothelial cells (SVECs) were cultured in Dulbecco’s modified eagle’s medium/Ham’s F-12 (PAA, Invitrogen, Karlsruhe) supplemented with 5 % FCS (Sigma-Aldrich, Schnelldorf, Germany). Cells were cultured at 37 °C and 5 % CO2.