Enhancement of EPC recruitment by angiogenic factors/chemokines: prominent role for MIF and VEGF. a, b Static adhesion of eEPCs on an endothelial layer under normoxic (a) and hypoxic (b) conditions (*p < 0.05 vs. isotype control, n = 3). c Chemotactic migration of calcein-labeled EPCs under normoxic conditions (*p < 0.05 vs. control, n = 3). d Chemotactic migration of calcein-labeled eEPCs under normoxic (black bars) and hypoxic (white bars) conditions towards MIF, VEGF, CXCL12, and CXCL1 (*p < 0.05 vs. control, § p < 0.05 vs. normoxia, # p < 0.05 vs. MIF under hypoxia; n = 3). e Transmigration of EPCs through an endothelial layer induced by MIF, VEGF, CXCL12, and CXCL1 (*p < 0.05 vs. control, n = 3). f Transmigration experiment as in e except that eEPCs were analyzed and normoxic versus hypoxic conditions compared for all chemokines/factors (*p < 0.05 vs. control, § p < 0.05 vs. normoxia, # p < 0.05 vs. MIF in hypoxia; n = 3)