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. 2002 Dec 9;72(1):101–114. doi: 10.1086/345489

Figure 3.

Figure 3

Rescue of COX content and activity in patient fibroblasts. Mitoplasts (20 μg protein) isolated from patient (N) and control (C) fibroblasts and patient and control fibroblasts overexpressing COX15 (N+COX15 and C+COX15, respectively) were analyzed by BN-PAGE. A, Western blot analysis using antibodies against COX subunits I and IV and against the 70-kDa subunit of Complex II (Co II) and the Core1 protein of Complex III (Co III). Overexpression of COX15 rescues COX levels in patient mitochondria. B, In-gel activity staining of COX and Complex I demonstrating restoration of COX activity in patient fibroblasts overexpressing COX15. The migration of molecular mass standards is indicated on the left.