Table 1.
COX15 Primers
|
Sequence |
|||
| Region | Upstream(5′→3′) | Downstream(5′→3′) | Product Size(bp) |
| cDNA 1124bp (EX1→EX9)a | tgaaggggaggcagtatctg | cagtgagcaaagccaaggag | 1,153 |
| cDNA 457bp (EX3→EX5) | cctctcgatggtagattggcattt | cggagggagtagcagtgacagtg | 457 |
| Exon 1 | gcctgcagtttccacttg | gggctctgatctgaacctat | 505 |
| Exon 2 | aaggctgatgcagtaatccagata | tcctcagtcaacctgtgcttctac | 402 |
| Exon 3/4 | atggcagctgtttctgacaa | aacaatgccaactaggaagctc | 837 |
| Exon 5 | caagatcccgccactg | gtccccatttaacgaacaat | 598 |
| Exon 6 | atggggtagaagggaaaaca | tgaagatgggggaatgaga | 429 |
| Exon 7 | ttggggtgggagcaggta | gtagggggacaggggtgaat | 564 |
| Exon 8 | gaagaggatggtggaaga | ttttgtagagatggggtttt | 474 |
| Exon 9 variant 1b | ggggaattttaggtttatca | tggcagacatttctttctc | 587 |
| Exon 9 variant 2c | gcccagctagttcctcttt | aggggttctggactcatcat | 704 |
| Intron 3/Exon4 | cttctaagcccccaaatccaaagtc | ttctgtcagtgtcatatcatgattcaaCctd |
192 |
a
Downstream primer is specific to exon 9 of the cox15 variant 1 mRNA transcript. This cDNA does not amplify 80 bp of 5′ and 64 bp of 3′ cDNA-coding sequence.
b
These primers are specific to COX15.1.
c
These primers are specific to COX15.2.
d
This primer has a mismatch at the underlined C (reference sequence=g).