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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Aug 13;1829(10):1147–1159. doi: 10.1016/j.bbagrm.2013.08.002

Figure 1. Effect of SRF depletion on the serum regulation of immediate early genes.

Figure 1

A) SRF levels in NIH3T3 cells stably expressing control shRNA or shRNA targeting SRF were measured by immunoblotting with anti-SRF sera. Anti-ERK1/2 antibodies were used as a loading control. B) Control and SRF shRNA cells were starved in 0.2% serum overnight and then serum induced with 20% NCS for the indicated times. The levels of the indicated genes were measured using quantitative real time-PCR (qPCR) with 18s rRNA levels used to normalize the samples. The values are the means of three experiments +/− standard deviations.