Figure 7. src inhibitor AZD0530 inhibits serum induction of Id1.

A) NIH3T3 cells were starved in 0.2% serum overnight and AZD0530 (100 nM) was added to the cells for 1 hour. Cells were then induced with 20% serum for the indicated times. Gene expression of indicated genes was analyzed by qPCR as in figure 1. B) The indicated luciferase reporters (described in figures 3 and 5) as well as a c-fos promoter reporter were transfected into NIH3T3 cells as in figure 3. The cells were treated with AZD0530 (100 nM) for 1 hour and then with 20% NCS for 4 hours. Luciferase assays were as in figure 3. *** indicates a p-value ≤ 0.05.