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. Author manuscript; available in PMC: 2013 Sep 29.
Published in final edited form as: Cell Rep. 2013 May 2;3(5):1580–1591. doi: 10.1016/j.celrep.2013.04.004

Table 1.

Generation of iPSC and iNP Colonies under Different Culture Conditions

Culture Conditions
 Number of iPSC Colonies (Day 20/30)
 Number of iNP Colonies (Day 20)
Temperature
 Medium
(Days 1–12) (Days 1–7) (Day 8) GM02504 GM03815 R47 GM02504 GM03815 R47
37°C CM CM 0/2 0/4 0/0 0 0 0
CDM 0/0 0/0 0/0 0 0 0
CDM CM 0/0 0/0 0/0 0 0 0
CDM 0/0 0/0 0/0 30 19 78
39° C CM CM 0/0 0/0 0/0 0 0 0
CDM 0/0 0/0 0/0 0 0 0
CDM CM 0/0 0/0 0/0 0 0 0
CDM 0/0 0/0 0/0 25 22 57
37°C FM CM+MEF 89/NA 157/NA 0/0 0 0 0

A total of 30,000 fibroblast cells were infected with SeV (OSKM) and cultured with different media (CM, CDM, or FM) for 1 week, then cells were reseeded onto laminin-coated or MEF-coated plates and cultured with different media (CM, CM, or CDM). CM, CM from γ-radiated MEFs cultured in human ESCM and with the treatment of 4 ng/ml FGF2; CM+MEF, cells reseeded onto MEF and cultured in CM; CDM, CDM with LSC; FM, fibroblast growth medium (DMEM with 10% FBS); NA, not analyzed.