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. Author manuscript; available in PMC: 2013 Sep 30.
Published in final edited form as: Cancer Prev Res (Phila). 2011 Nov 15;5(3):355–364. doi: 10.1158/1940-6207.CAPR-11-0299

Figure 6.

Figure 6

Reexpression of miR-26a decreased the expression of EZH2 and EpCAM proteins (A) and mRNAs (B) in MiaPaCa-2 cells and inhibited the mRNA expressions of EZH2, Oct4, Notch-1, and EpCAM in MiaPaCa-2 tumor sphere cells (C). Reexpression of let-7b and miR-26a decreased the formation of pancreatospheres of MiaPaCa-2 cells (D). The transfection of miR-26a miRNA precursors were conducted in MiaPaCa-2 cells and its tumor sphere cells as described under the Materials and Methods section. Western blot analysis and qRT-PCR were carried out to measure the relative protein levels and mRNA, respectively. The sphere forming assay was conducted as described under the Materials and Methods section. P values represent comparison between controls as calculated by the paired t test. Cont, control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Met, metformin.