Figure 4. Sin3B immunoreactivity is found outside the nucleus, where it colocalizes with immunostaining for Na_v channels A.

Discrete processes in the molecular layer of region CA1 of the hippocampus were labeled with anti-Sin3B (A20, green) as well as with a pan-specific antibody against Na_v channels (Pan, red). The middle panel shows superposition of the anti-Na_v-channel and anti-Sin3B staining. Images are single confocal optical sections. B. Extranuclear staining with anti-Sin3B (AK12, green) colocalized with immunostaining for Na_v1.2 (red). Arrows indicate neuronal processes where the two immunoreactivities coincide. Images are Z-axis projections of a series of 5 consecutive confocal optical sections taken in CA1; a single optical section is shown in the inset, for comparison. Note that immunostaining for Sin3B in panels A and B used two different anti-Sin3B antibodies.