Figure 6.

Intestinal stromal cells have a limited capacity for phagocytosis and antigen processing. (A) shows the quantification of FITC-labeled bead uptake by CD11c⁺ mDCs or CD90⁺ cultured iSCs, determined by flow cytometry at either 4°C (blue filled histograms) or 37°C (red open histograms), expressed as the fold increase in FITC MFI expression by cells at 37°C vs. 4^oC. (B) Shows quantification of antigen processing by CD11c+ mDCs or CD90+ iSCs, determined by flow cytometry after pulsing with DQ-OVA at either 4°C (blue filled histograms) or 37°C (red open histograms), expressed as the fold increase in FITC MFI expression by cells at 37°C vs. 4°C. Data in (A,B) show mean fold increases in FITC MFI by mDCs and iSCs from n = 3–5 patients. P values as indicated.