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. Author manuscript; available in PMC: 2014 Sep 12.
Published in final edited form as: Mol Cell. 2013 Aug 29;51(5):606–617. doi: 10.1016/j.molcel.2013.07.022

Figure 4. DRRRNA Promotes Myogenic Differentiation.

Figure 4

A. Immunofluorescence detection (red) of MyoD, MyoG, Myh in control, GFPi and DRRi cells. Note the reduction of MyoG and Myh in DRRi cells. B. Heat maps depicting the relative RNA levels (PolyA+ RNA-Seq) of myogenic genes in DRRi as compared to GFPi, and along side, relative transcript levels of myogenic genes from overexpressing cells (DRR1.2ox) as compared those expressing GFP (GFPox) are shown. Red arrows highlight MyoD and MyoG. C. Profiles of MyoD and MyoG occupancies as well as RNA (PE-Seq ribosome-depleted), the lengths of DRR fragments for overexpression are shown below. D. Increase in MyoG mRNA levels following overexpression of DRR1.2 and DRR2.0 fragments as compared to DRR0.5, a fragment −12kb upstream of MyoD (−12kb) and GFP. Biological replicates (n) and p-value are shown, data are represented as mean +/− SEM.