Fig. 5.

Increased migration activity of primary thyroid tumor cells induced by TGFβ1. (A) A wound healing assay was performed using primary cultured thyroid tumor cells isolated from thyroids of Thrb ^PV/PV mice (n = 3). Cells were treated (d, e, f, j, k and l) or not treated with TGFβ1 (a, b, c, g, h and i). An Olympus microscope at ×100 magnification was used to take images before and after treatment with TGFβ1 as indicated time. (B) The relative distance between cells was measured using Image J software, and data were analyzed by a two-way analysis of variance followed by Bonferroni post hoc tests. ***P < 0.001 when compared with WT; NS, not significant.