Fig. 3.

Effects of DAPM on AOM-induced colon carcinogenesis. A/J mice were treated with AOM as described in Materials and methods. Ten weeks after the last injection of AOM, mice were subjected to colonoscopic examination. After confirmation of tumors, mice were injected i.p. every other day with either DAPM or vehicle (control) for a total of 4 weeks. Mice were killed at week 20 (14 weeks after the last injection of AOM) and the frequency of tumors, and ACF was determined in whole-mount colons using a dissecting microscope. Size distribution of (A) tumors and (B) ACF in the colons harvested from control (n = 15) and DAPM-treated (n = 15) mice, respectively. Each data point represent an individual mouse, and the horizontal bars indicate the mean value and standard error for each group. Statistical analysis was performed using the Student’s t-test, *P < 0.05 compared with control mice.