Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jun 5;34(10):2330–2340. doi: 10.1093/carcin/bgt210

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Fig. 1. — Culture and characterization of MSCs from human HCC tissues. After collagenase digestion of surgical resected human HCC tissues and culturing, colonized cell clusters appeared (A) and these cells could rapidly grow and expand by subculture, showing typical fibroblast-like morphology (B). Using another method of culturing tiny tissue specimens, MSC-like cells could not be obtained from adjacent liver tissues (C) but only could be obtained from tumor tissues (D). (E) Adipogenic differentiation of liver carcinoma-derived MSCs, detected by Oil Red O staining for lipid droplets (arrow). (F) Osteogenic differentiation of these cells was evaluated by detection of deposited calcium phosphates using Alizarin Red S staining (arrow). (G) Fluorescence-activated cell sorting and staining confirmed that these cells are positive for the common mesenchymal markers CD13, CD73, CD105 and CD166 and are negative for the common hematopoietic markers CD34 and CD45. The percentages of cells positive for tumor-derived MSCs are presented as mean ± SD (n = 6).

Fig. 1. — Culture and characterization of MSCs from human HCC tissues. After collagenase digestion of surgical resected human HCC tissues and culturing, colonized cell clusters appeared (A) and these cells could rapidly grow and expand by subculture, showing typical fibroblast-like morphology (B). Using another method of culturing tiny tissue specimens, MSC-like cells could not be obtained from adjacent liver tissues (C) but only could be obtained from tumor tissues (D). (E) Adipogenic differentiation of liver carcinoma-derived MSCs, detected by Oil Red O staining for lipid droplets (arrow). (F) Osteogenic differentiation of these cells was evaluated by detection of deposited calcium phosphates using Alizarin Red S staining (arrow). (G) Fluorescence-activated cell sorting and staining confirmed that these cells are positive for the common mesenchymal markers CD13, CD73, CD105 and CD166 and are negative for the common hematopoietic markers CD34 and CD45. The percentages of cells positive for tumor-derived MSCs are presented as mean ± SD (n = 6).