Skip to main content
. 2013 Oct 10;19(11):1149–1165. doi: 10.1089/ars.2012.4909

FIG. 4.

FIG. 4.

Effects of the DPPen and DPPro lead analogs on enzyme activities in HCT 116 and MCF-7 cells. Lysates of HCT 116 and MCF-7 cells treated with indicated concentrations of SG, DPPen, and DPPro analogs for ∼12 h were collected for enzyme activity and protein expression determination. TrxR (A), Trx (B), GR (C), and GPx (D) activities were expressed as a percentage of dimethyl sulphoxide control. All data points are means±SE of two to four independent experiments. *Statistically significant difference (p<0.05) in enzyme activity compared to control. (E) The cell lysates with equal protein were analyzed by Western blotting with indicated antibodies for TrxR and Trx expression. Western blot images are representative of at least two independent experiments.